Here's how to make Tylenol work way better Dose it correctly: 1,000 mg at a time, spaced every 8 hours. Smaller doses don't control pain as well. Take it Why you should avoid REGULAR acetaminophen use for babies and pregnancy 3 Little Plums Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram