Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione, Alcohol and drugs Acetylcysteine for Acetaminophen Poisoning New England Journal of Medicine